Comparison of the expression of specific cell surface epitopes on in vitro fertilized and parthenogenetic bovine embryos

Lipocortin-1 (LC-1; annexin-1) may mediate some anti-inflammatory actions of the glucocorticoids, probably after binding to specific cell surface binding sites. We have quantified LC-1 levels in bronchoalveolar lavage (BAL) fluid and cells collected from seven healthy volunteers before and after 7 days of treatment with an oral glucocorticoid, prednisolone (30 mg/day). Extracellular BAL LC-1 was higher and cellular LC-1 was lower after prednisolone than before [extracellular: before, median 98 ng/mg albumin (range 48–350 ng/mg albumin); after, 236 ng/mg albumin (19–414 ng/mg albumin); P < 0.05. Cellular: before, 23.3 ng/10(6) cells (14.6–26.9 ng/10(6) cells); after, 18.0 ng/10(6) cells (122–268 ng/10(6) cells); P < 0.05]. The distribution of LC-1 within BAL cells ex vivo (cell surface = 25%, cytosol = 50%, membrane = 25%) was unaffected by prednisolone treatment. However, in adherent cells that had been cultured for 4 h, 70–80% of the LC-1 was on the cell surface. In summary, prednisolone appears to promote cellular release of LC-1. The difference in distribution of cellular LC-1 in BAL cells ex vivo and in vitro may reflect adherence and/or activation.

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Precision Printing of Cells and Biomaterials Onto 3D Matrices

ASME 2007 International Manufacturing Science and Engineering Conference ◽

10.1115/msec2007-31023 ◽

2007 ◽

Cited By ~ 1

Author(s):

Thomas Boland ◽

Xiaofeng Cui ◽

Aditya Chaubey ◽

Timothy C. Burg ◽

Richard E. Groff ◽

...

Keyword(s):

Cell Surface ◽

Cell Activity ◽

Significant Loss ◽

Specific Cell ◽

Surface Contact Area ◽

Cell Surface Interactions ◽

Printing Techniques ◽

Specific Cell Surface

The long term goal of our work is to develop a fabrication technique that allows precision placement of cells inside biomaterial constructs. Such spatial and temporal control of the chemistry and pattern geometry can provide new insights into fundamental aspects of cell-surface interactions. For example, cellular development can be dramatically effected by constraining cells to spread over a specific cell-surface contact area. The cell and biomaterial printing techniques developed here may prove particularly useful for exploring the interactions of anchorage-dependent cells with their environment in vitro. Our recent studies addressed the simultaneous printing of endothelial cells and biomaterials. The studies further demonstrated that cells can be printed onto polymer scaffolds or fibers without significant loss of cell activity. A combination of these methods may result in the construction of vascularized tissue with mechanical properties approaching those of native tissue.

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Colony formation in vitro by leukemic cells in acute lymphoblastic leukemia (ALL)

Blood ◽

10.1182/blood.v52.4.712.712 ◽

1978 ◽

Vol 52 (4) ◽

pp. 712-718 ◽

Cited By ~ 28

Author(s):

SD Smith ◽

EM Uyeki ◽

JT Lowman

Keyword(s):

Bone Marrow ◽

Acute Lymphoblastic Leukemia ◽

Bone Marrow Cells ◽

Lymphoblastic Leukemia ◽

Lymphoid Cells ◽

Assay System ◽

Leukemic Cells ◽

Specific Cell ◽

Specific Cell Surface

Abstract An assay system in vitro for the growth of malignant lymphoblastic colony-forming cells (CFC) was established. Growth of malignant myeloblastic CFC has been previously reported, but this is the first report of growth of malignant lymphoblastic CFC. Established assay systems in vitro have been very helpful in elucidating the control of growth and differentiation of both normal and malignant bone marrow cells. Lymphoblastic CFC were grown from the bone marrow aspirates of 20 children with acute lymphoblastic leukemia. Growth of these colonies was established on an agar assay system and maintained in the relative hypoxia (7% oxygen) of a Stulberg chamber. The criteria for malignancy of these colonies was based upon cellular cytochemical staining characteristics, the presence of specific cell surface markers, and the ability of these lymphoid cells to grow without the addition of a lymphoid mitogen. With this technique, specific nutritional requirements and drug sensitivities can be established in vitro, and these data may permit tailoring of individual antileukemic therapy.

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